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KMID : 1041220120500020189
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Journal of Sericultural and Entomological Science
2012 Volume.50 No. 2 p.189 ~ p.193
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Molecular characterization of diapause-related genes in the bumblebee, Bombus ignitus
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Hwang Jae-Sam
Park In-Gyun
Yun Eun-Young
Jung Duck-Oung
Yoon Hyung-Joo
Lee Sang-Beom
Kim Seong-Ryul
Lee Joon-Ha
Kim In-Woo
Kwon Young-Nam
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Abstract
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To find diapause-related genes, we were performed by differential hybridization with three types of [α-32P]dCTP-labeled total cDNA probes synthesized from diapause-prepared, diapause-maintained and diapause-activated stage of Bombus ignitus queen. Nine individual cDNA clones were found to be differentially expressed in diapause-maintained and diapause-activated stage. Among these clones, BIDC9(BIDC ; Bombus ignitus differentially expressed clone) was analyzed through full-length sequencing and expression pattern analysis. This clone was specifically expressed in the thorax organ. The effect of Juvenile hormone analog(JHA) and CO2 treatment was examined. JHA treatment induced the expression of BIDC9 cDNA clone abruptly after 4 day of treatment. CO2 treatment induced also the clone after 2 day of treatment. BIDC9 cDNA was identified as Bombus ignitus diapause gene contained an open reading frame of 1376 bp encoding 255 amino acids.
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KEYWORD
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Bombus ignitus, Diapause-related gene
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